This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation total DNA from tissue using economic salt out method |
| Applicazioni | PCR, enzyme digestion, Southern hybridization,eccetera. |
| Tecnologia di purificazione | Salting out method |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto animale |
| Importo del campione | Unlimited |
| Volume di eluizione | ≥100μl |
| Tempo per corsa | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finalmente, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 E 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Vantaggi
- Sicuro – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Purezza elevata – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Contenuto del kit
| Contenuti | D331201 | D331202 | D331203 |
| Tempi di purificazione | 1 G | 5 G | 50 G |
| Proteinasi K | 330 ml | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 X 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| Soluzione di RNasi | 330 ml | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
Conservazione e stabilità
RNase Solution should be stored at 2-8°C upon arrival. Tuttavia, conservazione a breve termine (fino a 24 settimane) a temperatura ambiente (15-25°C) non influisce sulle loro prestazioni. I restanti componenti del kit possono essere conservati asciutti a temperatura ambiente (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Assicurarsi che tutti i tamponi siano a temperatura ambiente quando utilizzati.
Recensioni
Non ci sono ancora recensioni.