Panoramica del Prodotto
- Nile Red is a lipophilic dye commonly used to detect lipid droplets within cells.
- It belongs to the family of lipophilic phenoxazine fluorescent dyes.
- When combined with esters, triglycerides, various fatty acids, and other lipid substances, Nile Red emits fluorescence.
- Under excitation at a wavelength of 543nm, it exhibits intense orange fluorescence (with an emission wavelength of 598nm).
- Inoltre, it shows red fluorescence under ultraviolet light.
- Furthermore, it can also be used for protein staining.
Specifica
| Property | Value |
|---|---|
| CAS | 7385-67-3 |
| English Name | Nile Red |
| Synonym | Nile Blue A oxazone; Phenoxazone 9 |
| Unità | Bottiglia |
| Purezza | ≥98% |
| Formula molecolare | C20H18N2O2 |
| Peso molecolare | 318.37 |
| Aspetto | Green to black solid |
| Magazzinaggio | Polvere: 2-8℃, 2 anni; In solvent (mother liquid): -20℃, 1 mese; -80℃, 6 mesi |
| Solubilità | Soluble in DMSO |
| CE | EINECS 230-966-0 |
| MDL | MFCD00011639 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| PubChem CID | 65182 |
| SMILES | O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1 |
| Descrizione | Nile Red is a dye commonly used to detect intracellular lipid droplets. |
Preparation of Stock Solution
Preparare un 10 mM stock solution using DMSO. Per esempio: Dissolve 1 mg of Nile Red powder in 314.1 μL of DMSO.
Nota:
- Unused stock solution is recommended to be aliquoted and stored at -20°C to avoid repeated freeze-thaw cycles.
- Moisture-absorbed DMSO significantly affects the solubility of the product. Please use freshly opened DMSO.
Preparation of Working Solution
Dilute the stock solution with an appropriate buffer (per esempio., serum-free culture medium or PBS) to prepare a working solution of 1-100 μm. Nota:
- The final concentration of the working solution is recommended to be optimized according to different cell lines and experimental systems.
- If difficult to dissolve, ultrasound treatment can be used to facilitate dissolution.
Precauzioni
- Avoid using lipid substances in cell culture media. During staining incubation and observation, avoid strong light exposure to prevent fluorescence quenching.
- Per la tua sicurezza e salute, wear lab coat and disposable gloves during operation.
- This product is for research use only. Do not use for pharmaceuticals, clinical diagnosis or treatment, food and cosmetics, eccetera. Do not store in residential areas.
Recensioni
Non ci sono ancora recensioni.