MagPure A3 Features:
- Technology Base:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of PCR amplicons.
- High-Throughput Compatibility:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
AmPure Technology:
- Selective Binding:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- Rimozione dei contaminanti:
- Efficiently removes excess primers, nucleotides, salts, and enzymes through a simple washing procedure.
- Purity of PCR Product:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
| Applicazioni | Sequencing, gene chip and qPCR, eccetera. |
| Tecnologia di purificazione | Tecnologia delle perle magnetiche |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | PCR products, enzymatic reaction solution |
| Importo del campione | Adeguata |
| Volume di eluizione | ≥10μl |
| Tempo di operazione | ≤50 minutes |
Principio
Product Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Rimozione dei contaminanti:
- Excess primers, nucleotides, salts, and enzymes are removed via a simple washing procedure.
- Eluizione del DNA:
- Purified DNA is eluted using Elution Buffer or Water.
Vantaggi
- Wide application – DNA products, enzyme digestion system, PCR reaction
- Recupero elevato – suitable for 100bp-20kb fragments
- Purezza elevata – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
Contenuto del kit
| Contenuti | BXP-5 | BXP-50 | BXP-500 |
| MagPure A3 | 5 ml | 50 ml | 500 ml |
Conservazione e stabilità
Storage and Handling Instructions:
- Condizioni di archiviazione:
- Store MagPure A3 at 2-8°C upon arrival.
- Stable for up to 18 mesi in queste condizioni.
- Short-term Storage:
- Short-term storage (fino a 12 settimane) a temperatura ambiente (15-25°C) does not affect performance.
- Preparazione del reagente:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogenous and consistent.
DO NOT FREEZE.
Recensioni
Non ci sono ancora recensioni.