introduzione
This product offers a fast and reliable method to isolate RNA, including miRNA, from a wide range of samples:
- Tessuto
- Cellule
- Sangue
- Other clinical samples
The extracted RNA is suitable for various downstream applications, Compreso:
- RT-PCR (Reverse Transcription PCR)
- Quantitative RT-PCR (qRT-PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| Applicazioni | RT-PCR, Sintesi di cDNA, second-generation sequencing |
| Prodotti | RNA, miRNA |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Clinical tissues, cellule, linfociti |
| Importo del campione | Tessuto: <20 mg
Cellule: <5 X 106 |
| Prodotto | 2-50MG |
| Volume di eluizione | ≥30μl |
| Tempo per corsa | ≤25 minutes |
Principio
- Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- Lisi e digestione del campione: The sample lysis process, coupled with proteinase K digestion, takes only 15 minuti, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- Elution: Finalmente, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
Vantaggi
- Efficient DNA removal – One-step Estrazione dell'RNA can effectively remove genomic DNA
- Alta qualità – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Veloce – the whole extraction only takes 15-25 minuti
- Nontoxic – no toxic phenol-chloroform extraction is required in the extraction
Contenuto del kit
| Contenuti | IVD4121 |
| Tempi di purificazione | 50 Preparativi |
| HiPure DNA Mini Column Ⅱ | 50 |
| HiPure RNA Mini Columns | 50 |
| 2ml Provette di raccolta | 150 |
| Proteinasi K | 24 mg |
| Tampone di dissoluzione della proteasi | 1.8 ml |
| DNasi I | 600 ml |
| Tampone dnase | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Buffer RW2* | 20 ml |
| Nuclease Free Water | 10 ml |
Conservazione e stabilità
- Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. Short-term storage (fino a 8 settimane) a temperatura ambiente (15–25°C) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. Short-term storage (fino a 1 settimana) a temperatura ambiente (15–25°C) does not affect its performance.
- Remaining Kit Components Storage: I restanti componenti del kit possono essere conservati a temperatura ambiente (15–25°C) and remain stable for at least 18 mesi in queste condizioni.
Recensioni
Non ci sono ancora recensioni.