Tessuto HiPure&Kit maxi colonna del DNA del sangue

• Challenges in DNA Extraction from Blood Samples:
  1. High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
  2. Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomico, and circulating DNA, leading to potential loss during extraction.
  3. Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
• Existing Extraction Methods:
  • Phenol-chloroform extraction, salting out, eccetera.
  • These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
• Product Benefits:
  • Purificazione veloce e facile: Provides a rapid and straightforward method for total Purificazione del DNA.
  • Suitability: Purifies total DNA (genomico, virale, mitocondriale) from whole blood, tessuto, e cellule coltivate.
  • Applicazione: Ensures reliable DNA for PCR and Southern blotting assays.

Specifiche

Principio

1. Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
2. Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
3. Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
4. DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.

Vantaggi

• High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, ibridazione, eccetera.
• Veloce – without separation of leukocytes, organic extraction, or ethanol precipitation
• Semplice – all nucleic acids can be obtained by direct digestion
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• Wide applicability – handle a variety of liquid samples
• Economia – excellent cost-effectiveness performance

Contenuto del kit

Conservazione e stabilità

Storage Recommendations:

• Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (fino a 12 settimane) a temperatura ambiente (15-25°C) is acceptable without affecting performance.
• Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 mesi in queste condizioni.

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