- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 minuti.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of colonna rotante tecnologia.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, digestione restrittiva, and next-generation sequencing.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 piatto |
| Applicazioni | PCR, Southern blot e digestione enzimatica, eccetera. |
| Metodo di purificazione | 96 bene piatto |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Soil, stool, other environmental samples |
| Importo del campione | Soil: 200-500mg
Sgabello: 50-100mg Other environmental samples: 100-500mg |
| Volume di eluizione | |
| Tempo per corsa | |
| Volume di trasporto del liquido per colonna | |
| Resa legante della colonna |
Principio
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, lievito, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteine, polysaccharides, and other contaminants from the lysed sample.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
Vantaggi
- Veloce – possono essere estratti diversi campioni 40 minuti (dopo la digestione)
- Purezza elevata – the purified DNA can be directly used in various downstream applications
- Buona ripetibilità – La tecnologia di silice può ottenere risultati ideali ogni volta
- Recupero elevato – Il DNA può essere recuperato a livello di PG
Contenuto del kit
| Contenuti | D314401 | D314402 | D314403 |
| Tempi di purificazione | 1 X 96 Preparativi | 4 X 96 Preparativi | 20 X 96 Preparativi |
| Piastra DNA HiPure | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6Piastra di raccolta ml | 1 | 4 | 20 |
| 0.8Piastra di raccolta ml | 1 | 4 | 20 |
| 2ml Tubi a perline | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 X 900 ml |
| Respingente Scheda di sicurezza | 10 ml | 36 ml | 180 ml |
| Reagente DX | 1 ml | 1.8 ml | 9 ml |
| Tampone ps | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| PIL tampone | 150 ml | 500 ml | 3 X 900 ml |
| Buffer GW2* | 50 ml | 100 ml | 4 X 200 ml |
| Buffer ae | 30 ml | 120 ml | 500 ml |
Conservazione e stabilità
- Absorber Solution Storage: Upon arrival, the Absorber Solution should be stored at 2-8°C. Tuttavia, it can also be stored at room temperature (15-25°C) for short-term periods (fino a 24 settimane) without affecting its performance.
- Remaining Kit Components: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 mesi in queste condizioni, ensuring their efficacy and performance over an extended period.
Recensioni
Non ci sono ancora recensioni.