introduzione
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA colonna rotante to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Co-isolation DNA and RNA from a single sample (cellule, soft tissue, plant sample) |
| Applicazioni | RT-PCR, Sintesi di cDNA, PCR andsecond-generation sequencing, eccetera. |
| Metodo di purificazione | Mini spin column |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Importo del campione | Soft Tissue: < 30mg, Cella: 1 X 107, Plant: 50 – 100mg |
| Prodotto | DNA: 2 – 20 μg, RNA: 5 – 50 μg |
Principio
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Vantaggi
- Alta qualità – RNA totale ad alta purezza / DNA can be directly used in a variety of downstream applications
- Veloce – column method can complete the extraction of several samples in 30 minuti
- Sicuro – Non è richiesta alcuna estrazione di cloroformio di fenolo
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Contenuto del kit
| Contenuti | R511102 | R511103 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Provette di raccolta | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer RW1 | 30 ml | 150 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| Acqua libera da RNasi | 10 ml | 30 ml |
| Buffer ae | 10 ml | 50 ml |
Conservazione e stabilità
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) e sono stabili almeno per 18 mesi in queste condizioni.
Recensioni
Non ci sono ancora recensioni.