Kit Midi Spin per DNA circolante HiPure D (Protocollo di rotazione)

introduzione

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:

• Targets:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Types:
  • Human plasma
  • Human serum
  • Human urine (fresh or frozen once)
• Focus on Short DNA: Captures short nucleic acids (<1000p.b) commonly found in biofluids.
• Efficient Purification: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.

Benefici:

• Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• Ampia compatibilità del campione: Works with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.

Dettagli

Specifiche

Caratteristiche	Specifiche
Funzioni principali	Isolamento del DNA circolante da 1-5 ml di plasma, siero, body fluids by spin
Applicazioni	qPCR, liquid or solid chip analysis, ibridazione, and SNP detection, eccetera.
Metodo di purificazione	Mini colonna rotante
Tecnologia di purificazione	Tecnologia della silice
Metodo di processo	Manuale (centrifugation)
Tipo di campione	Siero, plasma, and other cell-free fluid samples
Importo del campione	1-5ml
Volume di eluizione	≥50 μl
Tempo per corsa	≤70 minutes
Volume di trasporto del liquido per colonna	4ml
Resa legante della colonna	1mg

Principio

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. Lavaggio: Wash buffers to remove leftover proteins and cellular debris.
4. Eluizione: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Vantaggi

• Alta resa – processo più ottimale, free DNA (>50p.b) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• Purezza elevata – low alcohol binding method, completely removing inhibitor and protein pollution
• Recupero elevato – DNA can be recovered at the level of PG by silica gel column purification

Contenuto del kit

Contenuti	D318203D
Tempi di purificazione	50 Preparativi
Buffer ACL	250 ml
Buffer ACB*	300 ml
Buffer DCW1*	22 ml
Buffer DCW2*	10 ml
Proteinasi K	540 mg
Tampone di dissoluzione della proteasi	30 ml
RNA portante	110 μg
Nuclease Free Water	20 ml
HiPure CFDNA Mini Columns	50
2 ml Provette di raccolta	100
Extender Tubes	50
Sealing O-Ringes	50
50ml Collection Tube	50
Support Tube	50

Conservazione e stabilità

• Proteinasi K & RNA portante: Fridge (2-8°C) for best results.
• Everything Else: Temperatura ambiente (15-25°C) is fine.
• Important: If storing cold, warm buffers before use.