Perkenalan
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, parafin-embedded (Ffpe) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Co-isolation DNA and RNA from FFPE tissue |
| Aplikasi | RT-PCR, sintesis cDNA, PCR and second-generation sequencing, dll.. |
| Metode pemurnian | Polydisperse silicon based magnetic beads (DNA)
Monodisperse carbonyl magnetic beads (RNA) |
| Teknologi pemurnian | Teknologi manik-manik magnetik |
| Metode proses | Manual atau otomatis |
| Instrumen adaptif | Nucleic acid extractor and pipetting workstation |
| Jenis sampel | FFPE slice, FFPE puncture sample, embedded tissue |
| Jumlah sampel | No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area |
| Menghasilkan | DNA: 1 – 10 μg, RNA: 1 – 25 μg |
Prinsip
Sampel dilisiskan dan dicerna di bawah aksi lisat dan protease. DNA/RNA dilepaskan ke dalam lisat. Setelah menambahkan partikel magnetik dan larutan pengikat, DNA akan teradsorpsi pada permukaan partikel magnetik, the supernatant contain RNA was collected and bind to MagBind Particles. Partikel yang teradsorpsi dicuci dengan larutan pencuci untuk menghilangkan protein dan pengotor, dicuci dengan etanol untuk menghilangkan garam, and finally DNA/RNA was eluted by Elution Buffer.
Keuntungan
- Kualitas tinggi – high purity total RNA / DNA can be directly used in various downstream applications
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
- Post digestion sorting – higher DNA and RNA yields
- High RNA yield – monodisperse carbonyl adsorption
Isi Paket
| Isi | R632701 | R632702 | R632703 |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan | 5 X 96 Persiapan |
| MagBind Particles | 1.1 ml | 2.5 ml | 11 ml |
| Partikel magpure n | 1.1 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 48 mg | 220 mg |
| Buffer Larut Protease | 3 ml | 10 ml | 15 ml |
| DPS buffer | 50 ml | 100 ml | 2 X 250 ml |
| Penyangga ATL | 20 ml | 30 ml | 120 ml |
| Buffer BST1 | 20 ml | 40 ml | 200 ml |
| Buffer BXW1* | 44 ml | 110 ml | 3 X 110 ml |
| Air Bebas RNase | 15 ml | 30 ml | 120 ml |
Penyimpanan dan Stabilitas
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15–25°C) and are stablefor at least 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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