Ikhtisar Produk
- Suitable for rapid DNA extraction from Ffpe sampel.
- Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
- High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
- Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of high pure total DNA from FFPE using high bind beads |
| Aplikasi | PCR dan deteksi DNA virus, dll.. |
| Teknologi pemurnian | Teknologi manik-manik magnetik |
| Metode proses | Manual atau otomatis |
| Jenis sampel | Paraffin-embedded tissue samples |
| Jumlah sampel | 1-6 slices of 10-20μm |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤60 menit |
Prinsip
Keuntungan
- Hasil tinggi – proses yang paling optimal, recovery up to 90%
- Ekonomi – kurang dari 50% dari harga Qiagen dan produk impor lainnya
- Kemurnian tinggi – DARI 260/280: 1.7-1.9, DARI 260/230: 1.5-2.0
Isi Paket
| Isi | D632301D | D632302D |
| Waktu Pemurnian | 48 Persiapan | 96 Persiapan |
| Partikel magpure n | 1.1 ml | 2.5 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Buffer Larut Protease | 3 ml | 6 ml |
| DPS buffer | 60 ml | 100 ml |
| Penyangga ATL | 15 ml | 30 ml |
| Buffer BST1 | 30 ml | 60 ml |
| Buffer BW1 | 13 ml | 44 ml |
| Penyangga Elusi | 15 ml | 30 ml |
Penyimpanan dan Stabilitas
- Proteinase K, RNase A, and MagPure Particles N should be stored at 2-8°C initially.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks doesn’t affect their performance.
- Remaining kit components can be stored at room temperature and remain stable for at least 18 bulan dalam kondisi ini.
Ulasan
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