This kit provides fast purification of high-quality RNA from cells, tisu, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, Poly A+ RNA (mRNA) pemurnian, perlindungan nuclease, dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 X 106 cell using two columns (gDNA removed column) |
| Aplikasi | RT-PCR, qRT-PCR, Hibridisasi Utara, urutan generasi kedua |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Animal soft tissue, sel yang dikultur, limfosit, simple plant tissue |
| Jumlah sampel | Sel:≤1x 107
Animal tissue sample: 1-20 mg Jaringan tanaman: 50-150 mg |
| Menghasilkan | 2-100μg |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤25 minutes（1-24 samples） |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 menit. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Keuntungan
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – beberapa sampel dapat diekstraksi 25 Metode menit demi kolom
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Peka – RNA dapat dipulihkan pada tingkat PG
Isi Paket
| Isi | R411102 | D411103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure DNA Mini Columns | 50 | 250 |
| Kolom Mini RNA HiPure | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Penyangga RW2* | 12 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Ulasan
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