Blood is a valuable source of DNA for clinical diagnostics and research, but traditional extraction methods present challenges:
- Safety Hazards: Bloodborne pathogens pose a risk of infection during extraction.
- DNA Loss: Red blood cell removal can eliminate desired DNA fractions (virus, circulating DNA).
- Impurities and Inhibitors: Blood components can interfere with downstream applications (PCR, Blot Selatan).
This product offers a novel DNA extraction solution that addresses these issues:
- Safe and Efficient: No red blood cell removal minimizes infectious waste and contamination risks.
- Comprehensive DNA Recovery: Purifies total DNA (genomik, mitokondria, virus, circulating) for a complete picture.
- Kompatibilitas sampel yang luas: Works with whole blood (fresh/frozen), plasma, serum, mantel buffy, dan sel yang dikultur.
- Aplikasi hilir: Purified DNA is suitable for PCR and Southern blotting.
This innovative method simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers in diagnostics, forensik, and medical research.
Catatan: This bulletin corrects the previous statement about sample compatibility. This method is not suitable for tissue samples.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Aplikasi | PCR, deteksi baut selatan dan virus, dll. |
| Metode pemurnian | Midi kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Jaringan, sel, darah, air liur, penyeka, blood spot, air mani, dan sampel klinis lainnya |
| Jumlah sampel | 0.2-2 ml |
| Volume elusi | ≥300μl |
| Waktu per lari | ≤80 minutes |
| Volume pembawa cairan per kolom | 4ml |
| Hasil pengikatan kolom | 1mg |
Prinsip
This product offers a streamlined method for purifying DNA from blood samples using silica column technology.
The Process:
- Lisis dan Pencernaan: The blood sample is treated with a lysate solution and protease to break open cells and degrade proteins, releasing DNA into the solution.
- Mengikat Silika: The lysate is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules, sementara protein dan kotoran lainnya melewatinya.
- Pencucian: Wash buffers remove unbound proteins and cellular debris that didn’t adhere to the silica.
- Elusi: A low-salt buffer (typically 10 mM Tris, pH 9.0, Dan 0.5 mM EDTA) is used to elute the purified DNA from the silica. Hal ini mengganggu interaksi antara DNA dan silika, memungkinkan pengumpulan DNA yang dimurnikan dalam tabung terpisah.
Keuntungan
- DNA berkualitas tinggi – Memenuhi berbagai aplikasi hilir, termasuk PCR, qPCR, pencernaan enzim, hibridisasi, dll..
- Cepat – tanpa pemisahan leukosit, ekstraksi organik, atau presipitasi etanol
- Sederhana – Semua asam nukleat dapat diperoleh dengan pencernaan langsung
- Penerapan yang luas – menangani berbagai sampel cair
Isi Paket
| Isi | D311302 | D311303 |
| Waktu Pemurnian | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml Tabung Koleksi | 40 | 200 |
| Penyangga ATL | 50 ml | 250 ml |
| Penyangga AL | 50 ml | 250 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Penyangga GW2* | 12 ml | 50 ml |
| RNase A | 20 mg | 90 mg |
| Proteinase K | 100 mg | 440 mg |
| Buffer Larut Protease | 10 ml | 30 ml |
| Penyangga AE | 20 ml | 120 ml |
Penyimpanan dan Stabilitas
Untuk kinerja optimal:
- Store Proteinase K and RNase A at 2-8°C (lemari es) Sesampainya disana.
Penyimpanan jangka pendek yang dapat diterima:
- Proteinase K and RNase A can be stored for up to 12 minggu pada suhu kamar (15-25°C) tanpa mempengaruhi kinerja mereka.
Komponen kit yang tersisa:
- Store all other components of the kit dry at room temperature (15-25°C).
- Mereka stabil setidaknya untuk 18 bulan dalam kondisi ini.
Catatan Penting:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
Panduan Pembelian
| Nama | TIDAK KUCING | Jumlah sampel | Leukocyte protocol* | Colum type | Volume elusi | Average yield | Waktu per lari |
| Kit Mini DNA Darah HiPure | D3111 | 10-200μl | 1ml | 2kolom ml | ≥20μl | 5-9μg/200μl | ≤30 menit |
| Jaringan HiPure&Kit MIDI DNA Darah | D3113 | 0.2-2ml | 10ml | 1.5kolom ml | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Jaringan HiPure&DNA DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15kolom ml | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Jaringan HiPure&DNA darah 96 Perlengkapan | D3117 | 1-200μl | 1ml | 96 piring sumur | 3-8μg/200μl |
