- Challenges in DNA Extraction from Blood Samples:
- High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
- Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomik, and circulating DNA, leading to potential loss during extraction.
- Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
- Existing Extraction Methods:
- Phenol-chloroform extraction, salting out, dll..
- These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
- Product Benefits:
- Pemurnian Cepat dan Mudah: Provides a rapid and straightforward method for total pemurnian DNA.
- Suitability: Purifies total DNA (genomik, virus, mitokondria) from whole blood, jaringan, dan sel yang dikultur.
- Aplikasi: Ensures reliable DNA for PCR and Southern blotting assays.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Aplikasi | PCR, deteksi baut selatan dan virus, dll. |
| Metode pemurnian | Maxi kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Jaringan, sel, darah, air liur, penyeka, bercak darah, air mani, dan sampel klinis lainnya |
| Jumlah sampel | 3-10ml |
| Volume elusi | ≥700μl |
| Waktu per lari | ≤90 minutes |
| Volume pembawa cairan per kolom | 4ml |
| Hasil pengikatan kolom | 5mg |
Prinsip
- Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
- Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
- Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
- DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.
Keuntungan
- DNA berkualitas tinggi – Memenuhi berbagai aplikasi hilir, termasuk PCR, qPCR, pencernaan enzim, hibridisasi, dll..
- Cepat – tanpa pemisahan leukosit, ekstraksi organik, atau presipitasi etanol
- Sederhana – Semua asam nukleat dapat diperoleh dengan pencernaan langsung
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Penerapan yang luas – menangani berbagai sampel cair
- Ekonomi – excellent cost-effectiveness performance
Isi Paket
| Contents10 | 10 persiapan | 50 perps |
| Waktu Pemurnian | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Tabung Koleksi | 20 | 100 |
| Penyangga ATL | 120 ml | 550 ml |
| Penyangga AL | 120 ml | 2 X 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Penyangga GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Proteinase K | 120 mg | 540 mg |
| Buffer Larut Protease | 12 ml | 50 ml |
| Penyangga AE | 30 ml | 120 ml |
Penyimpanan dan Stabilitas
Storage Recommendations:
- Proteinase K and RNase A: Sesampainya disana, simpan pada suhu 2-8°C. Penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) dapat diterima tanpa mempengaruhi kinerja.
- Komponen Kit yang Tersisa: Can be stored at room temperature (15-25°C) dan tetap stabil setidaknya untuk 18 bulan dalam kondisi ini.
Panduan Pembelian
| Nama | TIDAK KUCING | Jumlah sampel | Leukocyte protocol* | Colum type | Volume elusi | Average yield | Waktu per lari |
| Kit Mini DNA Darah HiPure | D3111 | 10-200μl | 1ml | 2kolom ml | ≥20μl | 5-9μg/200μl | ≤30 menit |
| Jaringan HiPure&Kit MIDI DNA Darah | D3113 | 0.2-2ml | 10ml | 1.5kolom ml | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Jaringan HiPure&DNA DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15kolom ml | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Jaringan HiPure&DNA darah 96 Perlengkapan | D3117 | 1-200μl | 1ml | 96 piring sumur | 3-8μg/200μl |
