Ikhtisar Produk
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Capable of purifying various types of DNA, including genomic, virus, and mitochondrial.
- Can purify DNA from diverse sources: jaringan, darah utuh, plasma, serum, mantel buffy, bone marrow, other body fluids, limfosit, dan sel yang dikultur.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolasi total DNA dari darah, jaringan, culture cells, mengusap, and blood spots using 96 plate |
| Aplikasi | PCR, deteksi baut selatan dan virus, dll. |
| Metode pemurnian | 96 piring sumur |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Darah, serum, plasma, milk, air liur, and other liquid samples and cultured cells |
| Jumlah sampel | |
| Volume elusi | |
| Waktu per lari | |
| Volume pembawa cairan per kolom | |
| Hasil pengikatan kolom |
Prinsip
- Utilizes silica column purification method.
- Sampel dilisiskan dan dicerna dengan lisat dan protease, melepaskan DNA ke dalam lisat.
- Lysate is transferred to an adsorption column.
- Asam nukleat teradsorpsi pada membran, while protein is not adsorbed and is removed through filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Keuntungan
- DNA berkualitas tinggi – Memenuhi berbagai aplikasi hilir, termasuk PCR, qPCR, pencernaan enzim, hibridisasi, dll..
- High throughput – 96 samples can be processed simultaneously
Isi Paket
| Isi | D311701 | D311702 |
| Waktu Pemurnian | 1 X 96 | 4 X 96 |
| Pelat gDNA HiPure | 1 | 4 |
| 96 well Plate (2.2ml) | 1 | 4 |
| 1.6ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Silicon Seal Tape | 1 | 4 |
| Seal Film | 5 | 25 |
| Penyangga ATL | 30 ml | 100 ml |
| Penyangga AL | 30 ml | 100 ml |
| Buffer DW1 | 60 ml | 250 ml |
| Penyangga GW2 | 50 ml | 2 X 100 ml |
| Proteinase K | 50 ml | 200 ml |
| Buffer Larut Protease | 5 ml | 15 ml |
| Penyangga AE | 30 ml | 120 ml |
Penyimpanan dan Stabilitas
- Proteinase K harus disimpan pada 2-8 ° C pada saat kedatangan.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- Komponen kit lainnya dapat disimpan pada suhu kamar (15-25°C) dan tetap stabil setidaknya untuk 18 bulan dalam kondisi ini.
Panduan Pembelian
| Nama | TIDAK KUCING | Jumlah sampel | Leukocyte protocol* | Colum type | Volume elusi | Average yield | Waktu per lari |
| Kit Mini DNA Darah HiPure | D3111 | 10-200μl | 1ml | 2kolom ml | ≥20μl | 5-9μg/200μl | ≤30 menit |
| Jaringan HiPure&Kit MIDI DNA Darah | D3113 | 0.2-2ml | 10ml | 1.5kolom ml | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Jaringan HiPure&DNA DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15kolom ml | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Jaringan HiPure&DNA darah 96 Perlengkapan | D3117 | 1-200μl | 1ml | 96 piring sumur | 3-8μg/200μl |
Ulasan
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