Ikhtisar Produk
- Offers fast and easy methods for purification of total DNA.
- Ensures reliable PCR dan Southern blotting.
- Capable of purifying various types of DNA, including genomic, virus, and mitochondrial.
- Suitable for purification from tissue and cultured cells.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total DNA from <25 mg tissue, culture cells, FTA card |
| Aplikasi | PCR, Southern Bolt virus detection, dll.. |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Animal tissue or cultured cells |
| Jumlah sampel | Jaringan hewan : <25mg, Sel yang dikultur : <5 X 106 |
| Volume elusi | ≥30μl |
| Waktu per lari | 30 – 60 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
- Utilizes silica column purification method.
- Sampel dilisiskan dan dicerna dengan lisat dan protease, melepaskan DNA ke dalam lisat.
- Lysate is transferred to an adsorption column.
- Asam nukleat teradsorpsi pada membran, sedangkan protein tidak diserap dan dihilangkan dengan filtrasi.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Keuntungan
- Pengulangan yang baik – suitable for extracting high-yield DNA from different types of tissue samples
- Kemurnian tinggi – can be used for downstream applications such as multiplex and quantitative PCR
- Fas t- simplified process, extracting several samples in 20 menit (setelah pencernaan)
- Keamanan – no phenol or chloroform extraction, no alcohol precipitation
Isi Paket
| Isi | D312102 | D312103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Penyangga ATL | 15 ml | 65 ml |
| Penyangga DL | 15 ml | 65 ml |
| Penyangga GW1 | 22 ml | 110 ml |
| Penyangga GW2 | 12 ml | 50 ml |
| RNase A | 10 mg | 50 mg |
| Proteinase K | 24 mg | 120 mg |
| Buffer Larut Protease | 5 ml | 15 ml |
| Penyangga AE | 15 ml | 60 ml |
| Kolom Mini gDNA HiPure | 50 | 2 X 125 |
| 2 ml Tabung Koleksi | 100 | 5 X 100 |
Penyimpanan dan Stabilitas
- RNase A dan Proteinase K harus disimpan pada suhu 2-8 ° C pada saat kedatangan.
- Short-term storage at room temperature (15-25°C) for up to 24 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
- Atau, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Ulasan
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