Perkenalan
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. RNA yang didapat bisa langsung digunakan di RT-PCR, Northern blot, poly-A + pemurnian, Perlindungan asam nukleat dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from 500 mg soil sample |
| Aplikasi | RT-PCR, Northern blot, poly-A + pemurnian, Perlindungan asam nukleat dan terjemahan in vitro |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Forest soil, grassland soil, mining area soil, sediment and other samples |
| Jumlah sampel | 500 mg |
| Volume elusi | ≥30μl |
| Waktu per lari | ≤60 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (seperti guanidin hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Akhirnya, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Keuntungan
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – extraction of several samples can be completed in 60 minutes by the column purification method
- Aman – no phenol-chloroform extraction required
- Peka – RNA can be purified at the level of PG
Isi Paket
| Isi | R418302 | R418303 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Kolom mikro Hipure RNA | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 500 |
| gDNA Filter Column | 50 | 250 |
| 2ml Beads Tubes | 50 | 250 |
| Buffer SOL | 30 ml | 150 ml |
| Penyangga SDS | 4 ml | 15 ml |
| Buffer PHC | 30 ml | 150 ml |
| Buffer GDP | 40 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2 * | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
Buffer PHC should be stored at 2–8°C upon arrival. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan kering pada suhu kamar (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Seluruh kit dapat disimpan pada suhu 2–8°C, Tapi dalam hal ini, Buffer harus dilarutkan sebelum digunakan. Pastikan semua buffer berada pada suhu ruangan saat digunakan.
Data Eksperimen
Ulasan
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