This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. Kit ini didasarkan pada teknologi pemurnian kolom silika gel, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 menit. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, dan terjemahan in vitro.
Detail
Isi Paket
| Isi | R415002 | D415003 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| Kolom Mini RNA HiPure | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 500 |
| TCEP (1M) | 0.29 G | 5 X 0.29 G |
| Buffer EP | 1.0 ml | 5.0 ml |
| Buffer PSL | 50 ml | 250 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Penyangga RW2* | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
Except TCEP, the kit components can be stored dry at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
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