Perkenalan
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, sel, tisu, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, Poly A+ RNA (mRNA) pemurnian, perlindungan nuclease, dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total RNA from <1g simple plant sample (including plant sample containing starch) without chloroform |
| Aplikasi | RT-PCR, qPCR, Hibridisasi Utara, urutan generasi kedua, nucleic acid protection, in vitro translation |
| Metode pemurnian | Midi kolom putar |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Plant leaves, roots, stems, fruits, jamur, dll.. |
| Jumlah sampel | 0.5-1 G |
| Volume elusi | ≥400μl |
| Waktu per lari | ≤40 minutes |
| Volume pembawa cairan per kolom | 4ml |
| Hasil pengikatan kolom | 1mg |
Prinsip
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 menit. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Keuntungan
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – medium isolation of several samples can be completed in 40 menit
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Peka – RNA dapat dipulihkan pada tingkat PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Isi Paket
| Isi | R415202 | D415203 |
| Waktu Pemurnian | 20 Persiapan | 100 Persiapan |
| HiPure DNA Midi Column II | 20 | 100 |
| HiPure RNA Midi Columns II | 20 | 100 |
| 15ml Tabung Koleksi | 40 | 200 |
| Buffer RLC | 120 ml | 550 ml |
| Buffer PRC1 | 120 ml | 550 ml |
| Buffer RW1 | 70 ml | 400 ml |
| Penyangga RW2* | 20 ml | 100 ml |
| Air Bebas RNase | 30 ml | 120 ml |
Penyimpanan dan Stabilitas
The Kit can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Pastikan semua buffer berada pada suhu ruangan saat digunakan. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Ulasan
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