The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, Poly A+ RNA (mRNA) pemurnian, perlindungan nuclease, dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total RNA from Paxgene RNA Tubes |
| Aplikasi | RT-PCR, qPCR, Hibridisasi Utara, NGS, nucleic acid protection, in vitro translation |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika, DNA filtration technology |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Blood in the preservation tube |
| Jumlah sampel | 2.5ml |
| Volume elusi | ≥30μl |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanol is added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. The remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.
Keuntungan
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – isolation of several samples can be completed in 40 menit dengan menggunakan metode pemurnian kolom
- Keamanan – tidak diperlukan ekstraksi fenol-kloroform
- Peka – lisis darah secara langsung, plasma, dan sampel lain tanpa pemisahan leukosit
Isi Paket
| Isi | R416802 | R416803 |
| Waktu Pemurnian | 10 Persiapan | 50 Persiapan |
| HiPure RNA Mini Columns I | 10 | 50 |
| gDNA Filter Mini Columns | 10 | 50 |
| 2ml Tabung Koleksi | 30 | 150 |
| Air Bebas RNase | 60 ml | 250 ml |
| Buffer MBR1 | 10 ml | 30 ml |
| Buffer MBR2 | 5 ml | 15 ml |
| Buffer RW1 | 15 ml | 60 ml |
| Penyangga RW2* | 6 ml | 20 ml |
| Proteinase K | 12 mg | 50 mg |
| Buffer Larut Protease | 1.8 ml | 5 ml |
| DNase I | 120 μl | 600 μl |
| Penyangga DNAse | 6 ml | 30 ml |
Penyimpanan dan Stabilitas
Proteinase K harus disimpan pada 2-8 ° C pada saat kedatangan. DNase I sebaiknya disimpan pada suhu -20°C. Namun, penyimpanan jangka pendek (DNase aku sampai 1 minggu, Proteinase k hingga 8 minggu) pada suhu kamar (15–25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Data Eksperimen
Ulasan
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