HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, pengurutan, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | DNA Clean Up from agarose Gel and Reactions |
| Aplikasi | PCR, NGS, labeling, ligation and enzyme digestion, dll.. |
| Metode pemurnian | Micro kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Agarose gel, PCR products, enzyme products |
| Jumlah sampel | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>Agarose gel: ≤300mg
DNA products: <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤300µl (<span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤5µg) |
| Recovery | 100-5000bp：85%, 5-10kb：60-70% |
| Volume elusi | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥10μl |
| Waktu per lari | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤30 minutes（1-24 samples） |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 20mg |
Prinsip
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Keuntungan
- High recovery efficiency – hingga 80% DNA recovery
- Low elution volume – elution volume can be as low as 10μl
- General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
- Fast – isolation can be completed in 10-15 minutes by column gel method
Isi Paket
| Isi | D211002 | D211003 |
| Waktu Pemurnian | 100 Persiapan | 250 Persiapan |
| Buffer GDP | 60 ml | 125 ml |
| Buffer DW1 | 40 ml | 90 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Penyangga Elusi | 20 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Tabung Koleksi | 100 | 250 |
Penyimpanan dan Stabilitas
The Kit should be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Jika ada endapan yang terbentuk di buffer, hangatkan pada suhu 37℃ hingga larut.
Ulasan
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