HiPure Circulating DNA Mini Kit, designed to efficiently purify short, cell-free DNA (cfDNA) molecules from human biological fluids:

• Target:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000bp) commonly found in biofluids.
• Pemurnian yang Efisien: The protocol allows for effective recovery of cfDNA from various biofluids.
• Ready for Downstream Use: Purified cfDNA is suitable for diverse applications, termasuk:
  • PCR and other amplification reactions
  • Next-generation sequencing (NGS) analyses

Manfaat:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Kompatibilitas sampel yang luas: Works effectively with various human biofluids, offering flexibility in sample selection.
• Streamlined Workflow: The miniaturized kit format allows for faster processing compared to larger kits.

This kit empowers researchers to study circulating DNA in various fields, including oncology, non-invasive prenatal testing (NIPT), and liquid biopsy applications.

Spesifikasi

Prinsip

This product utilizes silica gel columns for a rapid and reliable pemurnian DNA process.

1. Lisis dan Pencernaan: The sample undergoes treatment with a lysate solution and protease. This breaks open cells and degrades proteins, releasing DNA into the solution.

2. Binding to Silica Membrane: The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, sementara protein dan kotoran lainnya melewatinya.

3. Pencucian: Buffer pencuci dilewatkan melalui kolom, menghilangkan protein yang tidak terikat dan sisa-sisa sel yang tidak menempel pada silika.

4. Elusi: A low-salt buffer (typically 10 mM Tris, pH 8.0) is used to elute the purified DNA from the silica membrane. Hal ini mengganggu interaksi antara DNA dan silika, memungkinkan pengumpulan DNA yang dimurnikan dalam tabung terpisah.

Metode ini memberikan alat yang berharga bagi para peneliti yang bekerja di berbagai bidang yang memerlukan DNA murni untuk analisis, such as PCR, Blot Selatan, and next-generation sequencing.

Keuntungan

• Hasil tinggi – proses yang paling optimal, DNA bebas (>50bp) dapat diperoleh secara maksimal
• Konsentrasi tinggi – volume elusi rendah, memastikan konsentrasi asam nukleat yang tinggi
• Kemurnian tinggi – metode pengikatan alkohol rendah, sepenuhnya menghilangkan inhibitor dan polusi protein
• Pemulihan tinggi – DNA dapat diperoleh kembali pada tingkat PG dengan pemurnian kolom silika gel

Isi Paket

Penyimpanan dan Stabilitas

Here’s a quick guide on storing your DNA extraction kit to ensure it functions at its best:

Ideal Storage:

• Store Proteinase K and Carrier RNA in the refrigerator (2-8°C) upon arrival for maximum effectiveness.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components should be stored dry at room temperature (15-25°C).
• They will remain stable for at least 18 bulan dalam kondisi ini.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

By following these storage recommendations, you can guarantee your DNA extraction kit is always ready for use and delivers the best possible results.