Kit Mikro Asam Nukleat Beredar HiPure

Kit Mikro Asam Nukleat Beredar HiPure, is designed to efficiently purify short nucleic acids (<1000bp) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfDNA) and mRNA in blood
  • Fetal nucleic acids in maternal blood
  • Free-circulating cell-free RNA (cfRNA)
  • Viral nucleic acids
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Pemurnian yang Efisien: The kit isolates short nucleic acids present in biofluids.
• High Purity: Purified nucleic acids are free of proteins, nucleases, dan kontaminan lainnya.
• Aplikasi hilir: The eluted nucleic acids are ready for use in various downstream analyses, termasuk:
  • Amplification reactions (misalnya, PCR)
  • Long-term storage (-30°C to -15°C)

Manfaat:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Kompatibilitas sampel yang luas: Works effectively with various human biofluids.
• High-Quality Results: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, including oncology, non-invasive prenatal testing (NIPT), and viral diagnostics.

Spesifikasi

Prinsip

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:

1. Lisis dan Pencernaan:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. This releases DNA into the solution.

2. Binding to Silica Membrane:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, sementara protein dan kotoran lainnya melewatinya.

3. Pencucian:

• Buffer pencuci dilewatkan melalui kolom, menghilangkan protein yang tidak terikat dan sisa-sisa sel yang tidak menempel pada silika.

4. Elusi:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. Hal ini mengganggu interaksi antara DNA dan silika, allowing collection of purified DNA in a separate tube.

Metode ini memberikan alat yang berharga bagi para peneliti yang bekerja di berbagai bidang yang memerlukan DNA murni untuk analisis, such as PCR, Blot Selatan, and next-generation sequencing.

Keuntungan

• Pemurnian yang Efisien: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• DNA berkualitas tinggi: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, cocok untuk berbagai aplikasi hilir.
• Alur Kerja yang Disederhanakan: Protokol ini melibatkan langkah-langkah penanganan minimal dan sentrifugasi untuk ekstraksi yang mudah digunakan dan efisien.

Isi Paket

Penyimpanan dan Stabilitas

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (lemari es) upon arrival for best results.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be stored for up to 12 minggu pada suhu kamar (15-25°C) tanpa mempengaruhi kinerja mereka.

Komponen Kit yang Tersisa:

• Store all other components of the kit dry at room temperature (15-25°C).
• Mereka stabil setidaknya untuk 18 bulan dalam kondisi ini.

Important Notes:

• If storing the entire kit at 2-8°C, Pastikan buffer dilarutkan kembali sebelum digunakan.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.