HiPure Sirkulasi DNA Midi Spin Kit D (Protokol Putar)

Perkenalan

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:

• Target:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Types:
  • Human plasma
  • Human serum
  • Human urine (fresh or frozen once)
• Focus on Short DNA: Captures short nucleic acids (<1000bp) commonly found in biofluids.
• Pemurnian yang Efisien: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.

Manfaat:

• Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• Kompatibilitas sampel yang luas: Works with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), dan biopsi cair, ultimately aiding in patient diagnosis and management.

Detail

Spesifikasi

Prinsip

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Mengikat Silika: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. Pencucian: Wash buffers to remove leftover proteins and cellular debris.
4. Elusi: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

Metode ini memberikan alat yang berharga bagi para peneliti yang bekerja di berbagai bidang yang memerlukan DNA murni untuk analisis.

Keuntungan

• Hasil tinggi – proses yang paling optimal, DNA bebas (>50bp) dapat diperoleh secara maksimal
• Konsentrasi tinggi – volume elusi rendah, memastikan konsentrasi asam nukleat yang tinggi
• Kemurnian tinggi – metode pengikatan alkohol rendah, sepenuhnya menghilangkan inhibitor dan polusi protein
• Pemulihan tinggi – DNA dapat diperoleh kembali pada tingkat PG dengan pemurnian kolom silika gel

Isi Paket

Penyimpanan dan Stabilitas

• Proteinase K & RNA pembawa: Kulkas (2-8°C) untuk hasil terbaik.
• Everything Else: Suhu kamar (15-25°C) is fine.
• Important: If storing cold, warm buffers before use.