Perkenalan
HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:
- Target:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Types:
- Human plasma
- Human serum
- Human urine (fresh or frozen once)
- Focus on Short DNA: Captures short nucleic acids (<1000bp) commonly found in biofluids.
- Pemurnian yang Efisien: Effectively recovers cfDNA from various biofluids.
- Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.
Manfaat:
- Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
- Kompatibilitas sampel yang luas: Works with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), dan biopsi cair, ultimately aiding in patient diagnosis and management.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolasi sirkulasi DNA dari plasma 1-5ml, serum, body fluids by spin |
| Aplikasi | qPCR, analisis chip cair atau padat, hibridisasi, dan deteksi SNP, dll.. |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi) |
| Jenis sampel | Serum, plasma, dan sampel cairan bebas sel lainnya |
| Jumlah sampel | 1-5ml |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤70 minutes |
| Volume pembawa cairan per kolom | 4ml |
| Hasil pengikatan kolom | 1mg |
Prinsip
This product utilizes silica gel columns for efficient DNA extraction:
- Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Mengikat Silika: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
- Pencucian: Wash buffers to remove leftover proteins and cellular debris.
- Elusi: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.
Metode ini memberikan alat yang berharga bagi para peneliti yang bekerja di berbagai bidang yang memerlukan DNA murni untuk analisis.
Keuntungan
- Hasil tinggi – proses yang paling optimal, DNA bebas (>50bp) dapat diperoleh secara maksimal
- Konsentrasi tinggi – volume elusi rendah, memastikan konsentrasi asam nukleat yang tinggi
- Kemurnian tinggi – metode pengikatan alkohol rendah, sepenuhnya menghilangkan inhibitor dan polusi protein
- Pemulihan tinggi – DNA dapat diperoleh kembali pada tingkat PG dengan pemurnian kolom silika gel
Isi Paket
| Isi | D318203D |
| Waktu Pemurnian | 50 Persiapan |
| Penyangga ACL | 250 ml |
| Penyangga ACB* | 300 ml |
| Penyangga DCW1* | 22 ml |
| Penyangga DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Buffer Larut Protease | 30 ml |
| RNA pembawa | 110 μg |
| Air Bebas Nuklease | 20 ml |
| Kolom Mini CFDNA HiPure | 50 |
| 2 ml Tabung Koleksi | 100 |
| Extender Tubes | 50 |
| Sealing O-Ringes | 50 |
| 50ml Collection Tube | 50 |
| Support Tube | 50 |
Penyimpanan dan Stabilitas
- Proteinase K & RNA pembawa: Kulkas (2-8°C) untuk hasil terbaik.
- Everything Else: Suhu kamar (15-25°C) is fine.
- Important: If storing cold, warm buffers before use.
Ulasan
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