HiPure Circulating DNA Midi Kit, designed for efficient purification of short, cell-free DNA (cfDNA) molecules from human biofluids for clinical use:
- Target:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Compatibility:
- Human plasma
- Human serum
- Human urine
- Samples can be fresh or frozen (once only)
- Focus on Short DNA: The kit isolates short nucleic acids (<1000bp) commonly found in biofluids.
- Pemurnian yang Efisien: The protocol allows for effective recovery of cfDNA from various biofluids.
- Clinical Applications: The purified cfDNA is suitable for use in in vitro diagnostic (IVD) tests approved for clinical purposes.
Manfaat:
- Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
- Kompatibilitas sampel yang luas: Works effectively with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows for processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsy analyses, ultimately aiding in patient diagnosis and management.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolasi sirkulasi DNA dari plasma 1-5ml, serum, body fluids using vacuum protocol |
| Aplikasi | qPCR, analisis chip cair atau padat, hybridization and SNP detection, dll.. |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (kosong) |
| Jenis sampel | Serum, plasma and other cell-free fluid samples |
| Jumlah sampel | 1-5ml |
| Volume elusi | ≥50μl |
| Waktu per lari | ≤60 menit |
| Volume pembawa cairan per kolom | 4ml |
| Hasil pengikatan kolom | 1mg |
Prinsip
This product offers a fast and reliable method for purifying DNA using silica column chromatography.
- Lisis dan Pencernaan: The sample is treated with a solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Mengikat Silika: The lysate is transferred to a column. The silica membrane in the column captures DNA molecules, while impurities pass through.
- Pencucian: Wash buffers to remove unbound proteins and cellular debris.
- Elusi: A low-salt buffer elutes the purified DNA from the silica.
Metode ini memberikan alat yang berharga bagi para peneliti yang bekerja di berbagai bidang yang memerlukan DNA murni untuk analisis.
Keuntungan
- Hasil tinggi – proses yang paling optimal, DNA bebas (>50bp) dapat diperoleh secara maksimal
- Konsentrasi tinggi – volume elusi rendah, memastikan konsentrasi asam nukleat yang tinggi
- Kemurnian tinggi – metode pengikatan alkohol rendah, sepenuhnya menghilangkan inhibitor dan polusi protein
- Pemulihan tinggi – DNA dapat diperoleh kembali pada tingkat PG dengan pemurnian kolom silika gel
Isi Paket
| Isi | D318201C | D318202C |
| Waktu Pemurnian | 10 | 50 |
| Penyangga ACL | 50 ml | 250 ml |
| Penyangga ACB* | 60 ml | 300 ml |
| Penyangga DCW1* | 4.4 ml | 22 ml |
| Penyangga DCW2* | 5 ml | 10 ml |
| Proteinase K | 110 ml | 540 mg |
| Buffer Larut Protease | 10 ml | 30 ml |
| RNA pembawa | 110 μg | 110 μg |
| Air Bebas Nuklease | 10 ml | 20 ml |
| Kolom Mini CFDNA HiPure | 10 | 50 |
| 2 ml Tabung Koleksi | 20 | 100 |
| Tabung Ekstender | 10 | 50 |
| Konektor Vac | 10 | 50 |
Penyimpanan dan Stabilitas
- Keep it cool: Store Proteinase K and Carrier RNA in the fridge (2-8°C) untuk hasil terbaik.
- Room temp is okay (for a while): They can last at room temperature (15-25°C) for up to 12 weeks without issues.
- Everything else: Store other kit components dry at room temperature (15-25°C), good for at least 18 bulan.
- Important: If storing everything cold, remember to warm up the buffers before use.
Data Eksperimen
Ulasan
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