Perkenalan

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Detail

Spesifikasi

Prinsip

Biological samples are first lysed and homogenized in a highly denaturing guanidin isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Keuntungan

• Kualitas tinggi – one step Ekstraksi RNA reagent combined with silica gel column can obtain the highest concentration
• Cepat – beberapa sampel dapat diekstraksi 40 menit
• High applicability – samples including animals, tanaman, bakteri, sel, dll..
• Konsentrasi tinggi – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Aman – no phenol chloroform extraction and no use of Trizol reagent

Isi Paket

Penyimpanan dan Stabilitas

Proteinase K harus disimpan pada 2-8 ° C pada saat kedatangan. Namun, penyimpanan jangka pendek (hingga 12 minggu) pada suhu kamar (15-25°C) tidak mempengaruhi kinerja mereka. Komponen kit lainnya dapat disimpan pada suhu kamar (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.