The Kit provides fast purification of high-quality RNA from whole blood, sel, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, Poly A+ RNA (mRNA) pemurnian, perlindungan nuclease, dan terjemahan in vitro.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation of total RNA from 1-1.5ml whole blood |
| Aplikasi | qPCR / RT-PCR, analisis chip fase cair atau padat, hibridisasi, dan deteksi SNP |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika, acid phenol/guanidin extraction technology (Teknologi pra-perawatan MagZol) |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Fresh or frozen blood, mammalian blood |
| Jumlah sampel | ≤1.5 ml whole blood |
| Menghasilkan | 2-100μg |
| Volume elusi | ≥20μl |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100μg |
Prinsip
The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.
Keuntungan
- Kualitas tinggi – RNA total dengan kemurnian tinggi dapat langsung digunakan dalam berbagai aplikasi hilir yang sensitif
- Cepat – isolation of several samples can be completed in 20 menit dengan menggunakan metode pemurnian kolom
- Peka – RNA can be purified at the level of PG
Isi Paket
| Isi | R416102 | R416103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 500 |
| 10 x Buffer RBC | 50 ml | 3 X 100 ml |
| Penyangga Lisis RTL | 50 ml | 250 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Penyangga RW2* | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
Penyimpanan dan Stabilitas
HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) dan setidaknya stabil 18 bulan dalam kondisi ini. Pastikan semua buffer berada pada suhu ruangan saat digunakan. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.
Ulasan
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