Perkenalan
Blood is a valuable source of DNA for various clinical applications and research studies. Namun, extracting DNA from blood samples presents several challenges:
Safety Concerns:
- Blood samples can harbor pathogens, posing a risk of infection to researchers and contaminating the lab environment.
- Traditional methods often involve hazardous chemicals like phenol-chloroform.
DNA Loss:
- Removing red blood cells, a common pre-treatment step can lead to the loss of desired DNA fractions like viral DNA, circulating DNA, and microbial DNA.
Impurities and Inhibitors:
- Blood contains various components that can interfere with downstream analyses like PCR dan Southern blotting.
New Technology for Improved Blood DNA Extraction:
This product offers a novel approach to address these challenges:
- Safe and Efficient: The method eliminates the need for red blood cell removal, minimizing infectious waste and potential contamination.
- Comprehensive DNA Recovery: The protocol allows for the purification of total DNA, including genomic, mitokondria, virus, and circulating DNA, providing a more complete picture for analysis.
- Kompatibilitas sampel yang luas: The method effectively purifies DNA from various blood components like whole blood (fresh or frozen), plasma, serum, mantel buffy, dan banyak lagi.
- Aplikasi hilir: The extracted high-quality DNA is suitable for reliable PCR and Southern blotting analyses.
This innovative solution simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers working in various fields like diagnostics, forensik, and medical research.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation total DNA from 200ul Whole Blood |
| Aplikasi | PCR, deteksi baut selatan dan virus, dll. |
| Metode pemurnian | Mini kolom putar |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Darah Utuh (fresh or frozen), serum, plasma, milk, air liur, and other liquid samples and cultured cells |
| Jumlah sampel | <200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells Non-mammalian animals that have a nucleus in red blood cells (rich in DNA, such as birds and fish): 5~20μl whole blood at a time. |
| Volume elusi | ≥20μl |
| Waktu per lari | ≤30 menit |
| Volume pembawa cairan per kolom | 800μl |
| Hasil pengikatan kolom | 100mg |
Prinsip
This method provides a reliable and convenient solution for researchers working in various fields that require purified DNA for analysis, such as PCR, Blot Selatan, and next-generation sequencing.
Efficient DNA Purification with Silica Column Technology
This product utilizes a silica column-based method for rapid and efficient DNA purification. Here’s a breakdown of the process:
- Sampel lisis dan pencernaan: The sample is treated with a lysate solution and protease to break down cell membranes and degrade proteins. This releases DNA into the solution.
- Binding to Silica Membrane: Lisat yang mengandung DNA dipindahkan ke kolom silika. The silica membrane in the column specifically binds DNA molecules through favorable interactions. Proteins and other impurities remain unbound.
- Pencucian: Buffer pencuci dilewatkan melalui kolom, removing unbound proteins and cellular debris that did not adhere to the silica membrane.
- Elusi: Akhirnya, a low-salt buffer (typically 10 mM Tris, pH 9.0, Dan 0.5 mM EDTA) is used to elute the purified DNA from the silica membrane. This buffer disrupts the interaction between DNA and the silica, allowing the purified DNA to be collected in a separate tube.
Keuntungan
- DNA berkualitas tinggi – Memenuhi berbagai aplikasi hilir, termasuk PCR, qPCR, pencernaan enzim, hibridisasi, dll..
- Cepat – tanpa pemisahan leukosit, ekstraksi organik, atau presipitasi etanol
- Sederhana – Semua asam nukleat dapat diperoleh dengan pencernaan langsung
- Penerapan yang luas- menangani berbagai sampel cair
Isi Paket
| Isi | D311102 | D311103 |
| Waktu Pemurnian | 50 | 250 |
| Kolom Mini Hipure DNA i | 50 | 2 X 125 |
| 2ml Tabung Koleksi | 100 | 5 X 100 |
| Penyangga AL | 15 ml | 60 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Penyangga GW2* | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Buffer Larut Protease | 1.8 ml | 10 ml |
| Penyangga AE | 15 ml | 60 ml |
Penyimpanan dan Stabilitas
Untuk kinerja optimal:
- Store Proteinase K at 2-8°C (lemari es) Sesampainya disana.
Penyimpanan jangka pendek yang dapat diterima:
- Proteinase K can be stored for up to 12 minggu pada suhu kamar (15-25°C) without affecting its performance.
Komponen kit yang tersisa:
- Store all other components of the kit dry at room temperature (15-25°C).
- Mereka stabil setidaknya untuk 18 bulan dalam kondisi ini.
Catatan Penting:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
