White Spot Syndrome Virus（WSSV）Nucleic Acid Test Kit (PCR-fluorescentna metoda sonde) 24T

Ovaj proizvod treba biti prepun vrećica za led i otpremljen kroz FedEx ili UPS.
Stići će unutar 7-10 dana. Naknada za dostavu uključena je u cijenu proizvoda.

Predstavljanje proizvoda：

This test kit offers a rapid and accurate method for detecting White Spot Syndrome Virus (WSSV) in shrimp.

• Tehnologija: Employs real-time fluorescent quantitative PCR (TaqMan probe method) for sensitive and specific detection.
• Targets: Utilizes specific primers and fluorescent probes designed to target the unique nucleic acid sequence of WSSV.
• Prijave: Detects WSSV in various shrimp samples, uključujući:
  • Animal tissues
  • Feed
  • Other sample types (consult kit specifications)
• Dual Detection: Provides qualitative and quantitative results:
  • Qualitative: Determines the presence or absence of WSSV.
  • Quantitative: Measures the amount of WSSV nucleic acid present, allowing for assessment of viral load.
• Real-Time Monitoring: Utilizes real-time PCR for continuous monitoring of amplification throughout the reaction, enabling rapid results.

This user-friendly test kit provides a valuable tool for shrimp farmers, researchers, and diagnostic labs for effective WSSV detection and management.

Sadržaj proizvoda：

Komponente	WP2101005-01（24T）
WSSV reaction solution	23μL per well × 8 wells per strip × 3 trake
WSSV positive control	100μL
negativna kontrola	100μL

Skladištenje:

Store at -20°C, with a shelf life of at least 12 mjeseca.

Applicable instrument：

ABI series (ABI 7300/ABI 7500/Step One, itd.), Roche LightCycler series, Bio-Rad CFX 96, SanshiBio Q162D, and other real-time fluorescence quantitative PCR instruments

Obrada uzorka：

Process the samples according to relevant standards, and store the processed samples for later use.

Experimental procedures：

• Priprema uzorka (Prostor za pripremu uzorka)

Please refer to the instruction manual of the total DNA/RNA extraction kit, or use the ES08 fully automated nucleic acid extraction and purification instrument with a matching rapid nucleic acid extraction kit (magnetic bead method), or other nucleic acid extraction kits/methods that comply with relevant standards to extract nucleic acid from the processed samples. The extracted sample nucleic acid should be tested as soon as possible and stored in an icebox, or else stored at -20°C.

• Priprema sustava za reakciju (Adding Area)

2.1 Take out the WSSV positive control tube, negative control tube, and reaction tubes containing the WSSV reaction solution required for the experiment (n+2) (Tj., n samples to be tested + pozitivna kontrola + negativna kontrola), completely thaw the reagents at room temperature, centrifuga za 10 sekundi, centrifuge the liquid from the tube wall and lid to the bottom of the tube, and store in an icebox for later use.

2.2 Then add 2μL of extracted negative control, sample nucleic acid, and WSSV positive control to the reaction tube in the eight-row strip, close the tube lid, napraviti zapisnik, and make the total volume of each reaction 25μL. Temeljito promiješajte, centrifuga za 30 sekundi, and perform the amplification experiment on the real-time fluorescence quantitative PCR instrument.

• Fluorescent PCR Amplification (Područje pojačanja i analize proizvoda)

Preddenaturacija na 95°C za 3 minuta; denaturation at 95°C for 5 sekundi, žarenja i istezanja na 60°C za 30 sekundi, za 40 ciklusi, collect fluorescence signal at 60°C. Choose FAM as the fluorescence group, and None as the quencher group (for use with ABI series real-time fluorescence quantitative PCR instruments, ako je potrebno, Unaprijed se obratite proizvođaču ili dodajte ROX kalibracijsku boju sami, and select ROX as the quencher group; inače, Slijedite normalan postupak).

• Result Determination

4.1 Ct value of the positive control < 30 i an “S” shaped amplification curve appears, the test result is valid. If not, Eksperiment bi trebao biti ponovljen. If the retest is still invalid, please contact the technical personnel.

4.2 Sample detection result: Ct vrijednost ≤ 38 i an “S” shaped amplification curve appears, indicating a positive result, which means the sample contains White Spot Syndrome Virus (WSSV).

4.3 Sample detection result: 38 < Ct < 40, it is considered suspicious. The sample should be retested. If the retest Ct value < 40 and there is a clear amplification curve, it is considered positive; inače, it is considered negative.

4.4 Sample detection result: Nema Ct vrijednosti ili Ct vrijednost ≥ 40 i br “S” oblikovana krivulja pojačanja, it is considered negative, indicating that White Spot Syndrome Virus (WSSV) was not detected in the sample.

Mjere predostrožnosti：

• This test kit has high sensitivity. Za sprječavanje kontaminacije, the experiment needs to be strictly partitioned, and physical isolation between partitions is recommended to avoid cross-contamination caused by human factors.
• Tijekom eksperimenta nosite laboratorijske kute i rukavice od lateksa. Use separate tools in different areas, and remember to change gloves and lab coats.
• The components in the reaction solution are light-sensitive and should be stored away from light. Prije upotrebe potpuno otopite reagense, ali izbjegavajte opetovano smrzavanje i odmrzavanje.
• Follow the instructions strictly for steps such as reagent preparation and sample addition. Disinfect the workbench, centrifuga, and pipettor regularly using chlorine disinfectants, etanol, sredstva za dekontaminaciju nukleinske kiseline, or ultraviolet light.
• After the reaction, place the PCR reaction tubes in sealed bags for disposal. Do not open the tubes to avoid aerosol contamination.
• Do not mix reagents from different batches. Use within the expiration date. Consumables should be treated without enzymes and sterile.
• A negative test result does not completely indicate a non-infected state; it is closely related to the quality of the obtained nucleic acid. “Negativan” results may be due to unqualified nucleic acid sample quality, low sample nucleic acid load, or unsuccessful nucleic acid extraction (otkrivanje). Veterinary clinical considerations are necessary for specific diagnoses.
• Possible causes of false positives: Cross-contamination during sample collection, transportation, and nucleic acid extraction.
• For any other questions, please contact the manufacturer’s technical staff promptly. Ovaj je proizvod namijenjen samo znanstvenim istraživanjima i nije namijenjen kliničkoj dijagnozi ili drugim svrhama.