Komplet za ispitivanje sadržaja Ferri iona
Bilješka: Uzmite dva ili tri različita uzorka za predviđanje prije testa.
Operativna oprema: Spektrofotometar
Cat Number: BC1730
Veličina: 50T/48s
Komponente:
Reagens i: Powder×2, storage at 4℃. Dodati 10 ML destilirana voda prije upotrebe.
Reagens ⅱ: Powder×2, storage at 4℃. Dodati 313 μL glacial acetic acid and 10 ML destilirana voda prije upotrebe. Standard Solution: Liquid 3 ml × 1, 1000 μmol/L Fe3+ standard solution, storage at 4℃. Add distilled water dilute 8 times to form a standard solution of 125 μmol/L before use.
Opis proizvoda:
Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia.
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 NM. According measure absorbance at 520 nm can reflect serum iron concentration.
Potrebni reagensi i oprema, ali nisu pruženi.
Spektrofotometar, centrifuga, glacial acetic acid, adjusted transferpettor, 1 ML staklena kiveta, chloroform and distilled water.
Postupak:
- Zagrijte spektrofotometar za 30 min, prilagoditi valnu duljinu na 520 NM, set zero with distilled
- Dilute standard solution to 125 μmol/L with distilled
- Dodajte reagense sa sljedećim popisom:
| Ime reagensa (μL) | Prazna cijev (Ab) | Epruveta (NA) | Standard (KAO) |
| Destilirana voda | 400 | ||
| Standard solution (125 μmol/L) | 400 | ||
| Serum(plazma) | 400 | ||
| Reagens i | 400 | 400 | 400 |
| Reagens II | 400 | 400 | 400 |
| Temeljito promiješajte, incubate in boiling water bath for 5 min, cooling liquid. Dodati 200 μL chloroform (required but not provided). Temeljito promiješajte, room temperature, 10000 rpm centrifuge for 10 min. uzeti 700 μL supernatant to 1 ML staklena kiveta. Measure absorbance at 520 NM. Recorded as AB, NA, KAO. | |||
Calculations
Serum iron (μmol/L)= =[Cs×(AT-AB)÷(As-AB)]= 125×(AT-AB)÷(As-AB)
Cs: Fe3+ standard solution, 125 μmol/L.
Bilješka:
- There is less iron in the serum, so the vessels (EP tubes) should be noted to avoid iron contamination.
- Reagent I and Reagent II are unstable. It needs to be prepared when the solution will be used, and the newly prepared reagent can only be used on the same day.
- When At- Ac＞5, please dilute the Serum to appropriate concentration with distilled water.
Technical Specifications：
Minimum Detection Limit：3.212 μmol/ml
Linear Range：3.9-250 μmol/ml
Povezani proizvodi：
BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) Komplet za analizu
BC0720/BC0725 Blood Calcium Content Assay Kit
Recenzije
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