Ovaj proizvod treba biti prepun vrećica za led i otpremljen kroz FedEx ili UPS.
Stići će unutar 7-10 dana. Naknada za dostavu uključena je u cijenu proizvoda.
Uvod
RNaza A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphategroup attached to the 3′-riboza susjednog pirimidin nukleotida. The resulting 2′,3′-cyclic phosphate is hydrolyzed to the corresponding 3′-nukleozidni fosfat. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 disulfidni mostovi.
A major application for RNase A is the removal of RNA from preparations of plazmidna DNK. The enzyme is active under a wide range of reaction conditions. Pri malim koncentracijama soli (0 do 100 MM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. Međutim, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Čistoća | ≥60% RNase A basis (SDS-STRANICA) |
| Enzymatic activity | >50 Kunitz jedinice/mg proteina |
| Optimum reaction temperature | 60°C (effective active temperature is 15-70°C) |
| Transportation conditions | Normal temperature transportation |
| Uvjeti očuvanja | -20-8°C, dry storage, long-term storage should be placed at -20°C. |
| Prijava 1: adding in the extraction process | 1. Plasmid Extraction: add RNase A (25mg/ml) to buffer P1 with a final concentration of 100-300μg/ml.
2. DNA extraction: add RNase A (25mg/ml) to the digestion solution with a final concentration is 100-400μg/ml, mix well and place at room temperature for 10-15 minuta. when SDS/CTAB in lysate exceeds 2%, RNase activity will be significantly inhibited; Guanidine salt(>4M guanidine hydrochloride or >3M guanidine isothiocyanate) also significantly inhibited RNase A. When RNase A is added to the lysate, the RNase digestion effect can be extracted by appropriately diluting to reduce the concentration of SDS, CTAB and guanidine salt. |
| Prijava 2: | 1. Remove RNA contamination from crude genomic DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10-100μg/ml. After mixing, place at room temperature for 10 minuta.
2. Remove RNA contamination from plasmid DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10μg/ml. After mixing, it can be directly used for sequencing at room temperature for 10 minuta. |
Informacije o naručivanju
| Sadržaj | C12123 | C12124 | C12128 | C12129 |
| RNase A Solution (25mg/ml) | 10 ml | 100 ml | ||
| DNase Free RNase A Solution (10mg/ml) | 10 ml | 100 ml |
Recenzije
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