Uvod
This product is suitable for the rapid extraction of DNA from tissue, stanice, krv, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA, and so on.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from blood, buffy coat, tissue, and other samples |
| Prijave | Second-generation sequencing, PCR, real-time PCR, itd. |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes, and cultured cells |
| Količina uzorka | Puna krv :< 200μl; Saliva / swab:< 400μl; Tkivo :< 20mg |
| Prinos | 0.1 – 50μg |
| Volumen eluacije | |
| Vrijeme po vožnji |
Načelo
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted by Elution Buffer.
Prednosti
- Visoka čistoća – OD 260 / 280: 1.7- 1.9, OD 260 / 230: 1.5 – 2.0
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Visok prinos – most optimal process, ensuring the recovery of up to 90%
- Strong processing ability – samples including animal blood, cultured cells, animal tissues, itd.
Sadržaj kompleta
| Sadržaj | IVD3102 |
| Vremena pročišćavanja | 200 |
| MagPure Particles | 5 ml |
| Proteinaza K | 100 mg |
| Pufer za otapanje proteaze | 10 ml |
| RNaza A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BW1* | 110 ml |
| Elucijski pufer | 30 ml |
Skladištenje i stabilnost
Proteinaza K, RNaza A, and MagPure Particles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
