This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minuta. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from tissue and cells and plant |
| Prijave | RT-PCR, cDNA synthesis, second generation sequencing |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Vrsta uzorka | Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue |
| Količina uzorka | Cells: 1 x 107
Animal tissue: ≤20mg Plant tissue: ≤100mg Yeast cell: ≤5 x 106 |
| Prinos | 5-100μg |
Načelo
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Prednosti
- Visoka čistoća – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Universal – suitable for various animal tissues, cultured cells and conventional plant fungal tissues
Sadržaj kompleta
| Sadržaj | R662201 | R662202 | R662203 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 5 x 96 Pripreme |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| Proteinaza K | 24 mg | 50 mg | 240 mg |
| Pufer za otapanje proteaze | 1.8 ml | 5 ml | 15 ml |
| DNaza I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase pufer | 30 ml | 40 ml | 200 ml |
| RTL Lysis Buffer | 40 ml | 80 ml | 400 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 x 220 ml |
| Međuspremnik RW2* | 20 ml | 50 ml | 2 x 100 ml |
| Voda bez RNaze | 10 ml | 30 ml | 120 ml |
Skladištenje i stabilnost
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. Međutim, kratkotrajno skladištenje (DNase I do 1 tjedni, MagPure RNA Particles and Proteinase K up to 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and are stable for atleast 18 mjeseci pod ovim uvjetima.
Recenzije
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