Uvod
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
pojedinosti
Načelo
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Sadržaj kompleta
| Sadržaj | R662601 | R662602 | R662603 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 5 x 96 Pripreme |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNaza I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase pufer | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 x 220 ml |
| Međuspremnik RW2* | 20 ml | 50 ml | 2 x 100 ml |
| Voda bez RNaze | 10 ml | 30 ml | 120 ml |
| 2ml Bead Tubes | 48 | 96 | 5 x 96 |
Skladištenje i stabilnost
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNazu I treba čuvati na -20°C. Međutim, kratkotrajno skladištenje (DNase I do 1 tjedni, MagPure RNA Particles up to 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 months under these
Recenzije
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