This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minuta. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from 250-500mg soil sample |
| Prijave | PCR, southern blot and enzyme digestion, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Soil |
| Količina uzorka | 250-500mg |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤70 minuta |
Načelo
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteini, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Prednosti
- High concentration – maximum extraction of total DNA from soil samples
- Visoka čistoća – purified DNA can be directly used in various downstream applications
Sadržaj kompleta
| Sadržaj | D635601 | D635602 | D635603 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 5 x 96 Pripreme |
| MagPure Particles | 2.5 ml | 5 ml | 22 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| Buffer SOL | 60 ml | 100 ml | 500 ml |
| Buffer SDS | 6 ml | 10 ml | 50 ml |
| Reagent DX | 1 ml | 1 ml | 5 ml |
| Buffer PS | 10 ml | 20 ml | 90 ml |
| Absorber Solution | 10 ml | 20 ml | 90 ml |
| Buffer GDP | 70 ml | 150 ml | 2 x 350 ml |
| Međuspremnik GW1* | 22 ml | 44 ml | 220 ml |
| Elucijski pufer | 20 ml | 20 ml | 60 ml |
Skladištenje i stabilnost
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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