MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minuta. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Pročišćena DNK može se koristiti izravno za PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija ukupne DNA iz 50-100 mg easy-grinded plant (tender leaf) and seed |
| Prijave | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Conventional economic plant samples |
| Količina uzorka | 50-100mg |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤60 minuta |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Prednosti
- Sef – require no phenol or chloroform extraction
- Brzo – može se izdvojiti nekoliko uzoraka 60 minuta
- Visoka čistoća – DNK visoke kvalitete, potpuno ukloniti inhibitore
Sadržaj kompleta
| Sadržaj | D635201 | D635202 | D635203 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 5 x 96 Pripreme |
| MagPure Particles | 1.7 ml | 4 ml | 18 ml |
| Buffer SOL | 40 ml | 90 ml | 500 ml |
| Buffer SDS | 4 ml | 9 ml | 50 ml |
| Buffer MPB | 40 ml | 70 ml | 350 ml |
| Buffer GW1 | 26 ml | 53 ml | 220 ml |
| Elucijski pufer | 10 ml | 30 ml | 120 ml |
Skladištenje i stabilnost
MagPure Particles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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