Uvod
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plazma, soaking solution, tissue homogenate supernatant, culture medium supernatant, itd. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) otkrivanje.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Main Function | Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plazma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection. |
| Prijave | Rt-PCR，PCR |
| Proizvodi | Pathogen RNA |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | cell-free/low-content cell biological samples such as body fluids, serums, plazma, tissue homogenate supernatant, sputum |
| Količina uzorka | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 40 minutes by column method
- Visoka kvaliteta – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Sef – no phenol chloroform extraction required
- Osjetljiv – DNA/RNA can be recovered at the level of PG
Sadržaj kompleta
| Sadržaj | IVD6672C |
| Vremena pročišćavanja | 200 Pripreme |
| 2ml Bead Tube | 4 x 50 |
| MagPure Particle | 7.0 ml |
| Proteinaza K | 100 mg |
| Pufer za otapanje proteaze | 6 ml |
| DTT （Powder) | 2g |
| Buffer SDS | 15 ml |
| Buffer MLBN | 220 ml |
| DNaza I | 4 x 0.6 ml |
| DNase pufer | 60 ml |
| Buffer MW1* | 53 ml |
| Buffer MW2* | 50 ml |
| Buffer NFW | 30 ml |
Skladištenje i stabilnost
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 2 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and are stable for 18 mjeseci pod ovim uvjetima.
Recenzije
Još nema recenzija.