Uvod
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples(including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plazma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS/tNGS application, remove host background nucleic acid. |
| Prijave | Real Time PCR, biochip analysis, NGS |
| Proizvodi | Pathogen DNA / RNA |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | whole blood, plazma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Količina uzorka | 0.5 – 1.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Sadržaj kompleta
| Sadržaj | R667200C | R6672-02C |
| Vremena pročišćavanja | 24 Pripreme | 96 Pripreme |
| 2ml Bead Tube | 24 | 96 |
| Buffer CLB2 | 10 ml | 40 ml |
| Proteinaza K | 12 mg | 50 mg |
| Pufer za otapanje proteaze | 1.8 ml | 6 ml |
| Buffer SDS | 1.5 ml | 10 ml |
| Reagent DX | 1.0 ml | 3.0 ml |
| Nuclease | 25 KU | |
| Nuclease Buffer | 1.5 ml | 5.0 ml |
| MagBind Particles | 1.0 ml | 3.5 ml |
| Buffer MLB | 15 ml | 60 ml |
| Buffer MW1* | 13 ml | 44 ml |
| Buffer MW2* | 10 ml | 50 ml |
| Buffer AVE | 10 ml | 20 ml |
Skladištenje i stabilnost
MagBind Particles, Nuclease and Proteinase K should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and are stable for 18 mjeseci pod ovim uvjetima.
Recenzije
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