This product is suitable for rapid extraction of DNA from FFPE uzorak, tissue, stanice, krv, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bps) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from FFPE using high bind beads |
| Prijave | Rt-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Large quantities of solids |
| Količina uzorka | Prikladno |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | 30 – 120 minuta |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Prednosti
- Visok prinos – most optimal process, recovery up to 90%
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Various operations – manual single tube/high-throughput or workstation
- Visoka čistoća – OD 260/280 : 1.7-1.9, OD 260/230 :1.5-2.0
Sadržaj kompleta
| Sadržaj | D632301B | D632302B |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme |
| MagBind Particles | 1.1 ml | 2 x 1.1 ml |
| RNaza A | 10 mg | 20 mg |
| Proteinaza K | 24 mg | 48 mg |
| Pufer za otapanje proteaze | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer AL | 15 ml | 30 ml |
| Buffer BD* | 6 ml | 15 ml |
| Buffer BXW1* | 13 ml | 44 ml |
| Elucijski pufer | 15 ml | 30 ml |
Skladištenje i stabilnost
RNaza A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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