Pregled proizvoda
- Suitable for rapid DNA extraction from FFPE uzorci.
- Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
- High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
- Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.
Tehnički podaci
Značajke | Tehnički podaci |
Glavne funkcije | Isolation of high pure total DNA from FFPE using high bind beads |
Prijave | PCR and viral DNA detection, itd. |
Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
Procesna metoda | Ručno ili automatski |
Vrsta uzorka | Paraffin-embedded tissue samples |
Količina uzorka | 1-6 slices of 10-20μm |
Volumen eluacije | ≥30μl |
Vrijeme po vožnji | ≤60 minuta |
Načelo
Prednosti
- Visok prinos – most optimal process, recovery up to 90%
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Visoka čistoća – OD 260/280: 1.7-1.9, OD 260/230: 1.5-2.0
Sadržaj kompleta
Sadržaj | D632301D | D632302D |
Vremena pročišćavanja | 48 Pripreme | 96 Pripreme |
MagPure Particles N | 1.1 ml | 2.5 ml |
RNaza A | 10 mg | 20 mg |
Proteinaza K | 24 mg | 48 mg |
Pufer za otapanje proteaze | 3 ml | 6 ml |
Buffer DPS | 60 ml | 100 ml |
Buffer ATL | 15 ml | 30 ml |
Buffer BST1 | 30 ml | 60 ml |
Buffer BW1 | 13 ml | 44 ml |
Elucijski pufer | 15 ml | 30 ml |
Skladištenje i stabilnost
- Proteinaza K, RNaza A, and MagPure Particles N should be stored at 2-8°C initially.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks doesn’t affect their performance.
- Remaining kit components can be stored at room temperature and remain stable for at least 18 mjeseci pod ovim uvjetima.
Recenzije
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