Magpure ffpe DNK komplet (High Pure)

Iz$149.00

Dostava USD 45 - Besplatno preko USD 300

DTE je kineska platforma za e-trgovinu specijalizirana za online prodaju molekularnog testiranja, ELISA, i srodni proizvodi.

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Opis

Pregled proizvoda

  • Suitable for rapid DNA extraction from FFPE uzorci.
  • Utilizes two combination methods: High-salt Bind and Alcohol mediated adsorption.
  • High-salt Bind removes pigments or polysaccharides from complex FFPE samples, enhancing nucleic acid purity and preventing blockages in aligent 2100.
  • Alcohol-mediated adsorption improves nucleic acid yield in high-yield samples.

Tehnički podaci

Značajke Tehnički podaci
Glavne funkcije Isolation of high pure total DNA from FFPE using high bind beads
Prijave PCR and viral DNA detection, itd.
Tehnologija pročišćavanja Tehnologija magnetskih kuglica
Procesna metoda Ručno ili automatski
Vrsta uzorka Paraffin-embedded tissue samples
Količina uzorka 1-6 slices of 10-20μm
Volumen eluacije ≥30μl
Vrijeme po vožnji ≤60 minuta

Načelo

  • Utilizes high-binding magnetic particles for purification.
  • The sample is lysed and digested with lysate and Protease.
  • DNA is released into the lysate during this process.
  • Magnetic particles and binding solution are added, leading to DNA adsorption on particle surfaces.
  • Impurities like proteins are not adsorbed and are removed.
  • Washed with washing solution to remove proteins and impurities.
  • Ethanol wash removes salts.
  • DNA is finally eluted using an Elution Buffer.

Prednosti

  • Visok prinos – most optimal process, recovery up to 90%
  • Economy – manje nego 50% of the price of Qiagen and other imported products
  • Visoka čistoća – OD 260/280: 1.7-1.9, OD 260/230: 1.5-2.0

Sadržaj kompleta

Sadržaj D632301D D632302D
Vremena pročišćavanja 48 Pripreme 96 Pripreme
MagPure Particles N 1.1 ml 2.5 ml
RNaza A 10 mg 20 mg
Proteinaza K 24 mg 48 mg
Pufer za otapanje proteaze 3 ml 6 ml
Buffer DPS 60 ml 100 ml
Buffer ATL 15 ml 30 ml
Buffer BST1 30 ml 60 ml
Buffer BW1 13 ml 44 ml
Elucijski pufer 15 ml 30 ml

Skladištenje i stabilnost

  • Proteinaza K, RNaza A, and MagPure Particles N should be stored at 2-8°C initially.
  • Short-term storage at room temperature (15-25°C) for up to 12 weeks doesn’t affect their performance.
  • Remaining kit components can be stored at room temperature and remain stable for at least 18 mjeseci pod ovim uvjetima.

Dodatne informacije

Težina 0.7 kg
veličina

Magpure ffpe DNK komplet (High Pure) 48 pripreme, Magpure ffpe DNK komplet (High Pure) 96 pripreme

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