Fast and Easy Total Pročišćavanje DNK
- Objective: Reliable PCR and Southern Blotting
- Značajke:
- Swift purification methods
- Easy-to-follow protocols
- Benefits:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Puna krv
- Plazma
- Serum
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Cultured cells
- Purifies various types of total DNA:
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from 200μl whole blood |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Puna krv, concentrated blood, buffy coat, and other samples |
| Količina uzorka | 200μl |
| Volumen eluacije | ≥70μl |
| Vrijeme po vožnji | ≤40 minuta |
Načelo
- Sample lysis and digestion facilitated by lysate and Protease.
- DNK se oslobađa u lizat.
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
Postupak:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: DNK se oslobađa u lizat.
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Washing:
- First, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Elution: DNA is eluted using an Elution Buffer.
Prednosti:
-
- High specificity: Selective DNA binding, excluding impurities.
- Efficiency: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Prijave:
-
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Prednosti
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, such as whole blood, buffy coat, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Second-generation sequencing (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Sadržaj kompleta
| Sadržaj | D631001 | D631002 | D631003 |
| Vremena pročišćavanja | 48 | 96 | 480 |
| MagPure Particles | 1.7 ml | 3.5 ml | 17 ml |
| Proteinaza K | 24 mg | 50 mg | 220 mg |
| Pufer za otapanje proteaze | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Međuspremnik DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Elucijski pufer | 15 ml | 30 ml | 100 ml |
Skladištenje i stabilnost
Proteinaza K, MagPureParticles should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje(do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C)i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
