MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Prijave | qPCR, NGS, itd. |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Serum, plazma |
| Količina uzorka | 0.6ml |
| Volumen eluacije | |
| Vrijeme po vožnji |
Načelo
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNK se oslobađa u lizat. After adding magnetic particles and binding solution, Large DNA(>500bps) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bps) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
Prednosti
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Automatic – without labour
Sadržaj kompleta
| Sadržaj | 1292750 | 12927200 |
| Vremena pročišćavanja | 50 pripreme | 200 pripreme |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinaza K | 24 mg | 90 mg |
| Pufer za otapanje proteaze | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Elucijski pufer | 15 ml | 60 ml |
Skladištenje i stabilnost
Proteinaza K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2-8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
Još nema recenzija.