This product supplies a simple and rapid extraction of total RNA from Blood, buffy coat, bone marrow, cell suspension, and other body fluids. The kit is based on superparamagnetic particle purification technology, with no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing, and so on.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from 300μl whole blood, bone marrow, buffy coat |
| Prijave | RT-PCR, cDNA synthesis, second-generation sequencing |
| Metoda pročišćavanja | Polydisperse magnetic beads |
| Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
| Procesna metoda | Ručno ili automatski |
| Vrsta uzorka | Puna krv, buffy coat, bone marrow, cultured cells, lymphocytes, body fluid |
| Količina uzorka | ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow |
| Prinos | 1-30μg |
Načelo
The Kit can be used for both manual extraction processes and automatic nucleic acid extraction machines. This Kit is suitable for extracting RNA from ≤5×106 cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. RNA/DNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally, RNA was eluted by Elution Buffer.
Prednosti
- Visoka čistoća – OD 260 / 280:1.9-2.0, OD 260 / 230:1.5-2.0
- Economy – manje nego 50% of the price of Qiagen and other imported products
- Automatic – extraction without manual participation, saving time and effort
- Visok prinos – nucleic acid recovery up to 95%
- Općenito – samples include whole blood, serum, plazma, lymphocyte suspension, bone marrow, cultured cells, itd.
Sadržaj kompleta
| Sadržaj | R661101 | R661102 | R661103 |
| Vremena pročišćavanja | 48 Pripreme | 96 Pripreme | 480 pripreme |
| MagPure RNA Particles | 1.7 ml | 3.5 ml | 18 ml |
| Proteinaza K | 24 mg | 50 mg | 220 mg |
| Pufer za otapanje proteaze | 1.8 ml | 5 ml | 15 ml |
| DNaza I | 600 μl | 2 x 600 μl | 10 x 600 µl |
| DNase pufer | 30 ml | 40 ml | 200 ml |
| Buffer MLB | 40 ml | 80 ml | 350 ml |
| Buffer MCB* | 15 ml | 30 ml | 150 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 x 100 ml |
| Voda bez RNaze | 10 ml | 15 ml | 120 ml |
Skladištenje i stabilnost
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNazu I treba čuvati na -20°C. Međutim, kratkotrajno skladištenje (DNase I do 1 week, MagPure RNA Particles and Proteinase K up to 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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