Uvod
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plazma, immersion solution, tissue homogenate supernatant, culture supernatant, itd., the extracted products can be used for clinical in vitro detection.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Extract viral RNA/DNA from 200μl plasma/serum samples |
| Prijave | Rt-PCR，PCR，NGS |
| Proizvodi | Viral DNA / RNA, body cell DNA / RNA |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Plazma, serum, effusion, urine, fecal suspension supernatant |
| Količina uzorka | 200μl |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | |
| Nosivi volumen tekućine po stupcu | 800μl |
| Prinos vezivanja kolone | 100μg |
Načelo
This product is based on silica gel purification. Uzorak se lizira i probavlja lizatom i proteazom, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, dok se protein ne adsorbira i uklanja se filtracijom. Nakon pranja proteina i drugih nečistoća, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Prednosti
- Brzo – column purification without PK digestion
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Sef – no phenol chloroform extraction required
- Osjetljiv – DNA/RNA can be recovered at the level of PG
Sadržaj kompleta
| Sadržaj | IVD4175 |
| Vremena pročišćavanja | 100 Pripreme |
| HiPure Viral Column | 100 |
| 2ml cijevi za prikupljanje | 100 |
| PK/Carrier RNA | 50 mg/310μg |
| Pufer za otapanje proteaze | 5 ml |
| Buffer VLE | 42 ml |
| Buffer CE | 60 ml |
| Voda bez RNaze | 15 ml |
Skladištenje i stabilnost
This kit is shipped and stored at room temperature and is valid for 12 mjeseca.
Recenzije
Još nema recenzija.