Uvod
The Kit integrates phenol/gvanidin-based lysis and silica-membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, biljka, and fungal samples, bacteria, stanice, and other samples.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total RNA (Uključujući miRNA) from 100mg lipid tissue, tissue, cell, biljka, and body fluids using columns and MagZol reagent |
| Prijave | Rt-PCR, qRT-PCR, Sjeverna hibridizacija, second-generation sequencing |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Animal tissue, muscle fiber-containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants, and other biological samples |
| Količina uzorka | Animal tissue sample: 1-60mg (spleen ≤ 10mg)
Cultured cells: 5 x 106 Plant leaves: 50-100mg |
| Prinos | 2-200μg |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤30 minutes（1-24 samples） |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Prednosti
- Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
- Visoka kvaliteta – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Brzo – može se izdvojiti nekoliko uzoraka 30 minuta
- High applicability – samples including animals, biljke, bacteria, stanice, itd.
- High output – enables to processing of large samples and the yield can be up to 200μg
Sadržaj kompleta
| Sadržaj | R413002 | D413003 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Međuspremnik RW1 | 50 ml | 200 ml |
| Međuspremnik RW2* | 20 ml | 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
MagZol Reagent should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njegovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Podaci o eksperimentu
Recenzije
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