This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA (mRNA) pročišćavanje, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Izolacija Ukupna RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column) |
| Prijave | RT-PCR, qRT-PCR, Sjeverna hibridizacija, second generation sequencing |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, Tehnologija filtracije DNA |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Količina uzorka | Cells:≤1 x 107
Animal tissue sample: 1-20 mg Plant tissue: 50-150 mg |
| Prinos | 2-100μg |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤25 minutes（1-24 samples） |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Prednosti
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Brzo – može se izdvojiti nekoliko uzoraka 25 minutes by column method
- Sef – no phenol chloroform extraction required
- Osjetljiv – RNA can be recovered at the level of PG
Sadržaj kompleta
| Sadržaj | R411102 | D411103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Međuspremnik RW1 | 50 ml | 200 ml |
| Međuspremnik RW2* | 12 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Recenzije
Još nema recenzija.