Uvod
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Recovery of single stranded or double stranded RNA from crude RNA products and enzymatic reaction solution |
| Prijave | Sequencing, ligation, enzyme digestion, RT-PCR, labeling, itd. |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Crude RNA, enzymatic reaction solution |
| Količina uzorka | ≤100μl |
| Oporavak | 90% |
| Volumen eluacije | ≥15μl |
| Vrijeme po vožnji | ≤15 minutes |
| Nosivi volumen tekućine po stupcu | 800μl |
| Prinos vezivanja kolone | 100μg |
Načelo
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Prednosti
- Visoki oporavak – recovery of RNA up to 90%
- Low elution volume – the elution volume can be as low as 15µl
- Brzo – only 10 minutes for recovery by using column method
Sadržaj kompleta
| Sadržaj | R214402 | R214403 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| Buffer GXP | 30 ml | 120 ml |
| Buffer RW2 | 20 ml | 2 x 50 ml |
| RNase-Free Water | 20 ml | 60 ml |
| HiPure RNA mini stupci I | 50 | 250 |
| 2 ml cijevi za prikupljanje | 50 | 250 |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2-8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
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