The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poli A+ RNA (mRNA) pročišćavanje, zaštita od nukleaze, i in vitro prijevod.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from <150 mg simple plant sample (including plant sample containing starch) without chloroform |
| Prijave | RT-PCR, qPCR, Sjeverna hibridizacija, second generation sequencing, zaštita nukleinske kiseline, in vitro prijevod |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija, Tehnologija filtracije DNA |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Economic crops |
| Količina uzorka | ≤150 mg |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤25 minutes |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Prednosti
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Brzo – može se izdvojiti nekoliko uzoraka 25 minutes by column method
- Sef – no phenol chloroform extraction required
- Osjetljiv – RNA can be recovered at the level of PG
- Široki spektar – various types of plant samples can be processed by diversity of operating procedures
Sadržaj kompleta
| Sadržaj | R415102 | D415103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure DNA Mini Column II | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer PRC1 | 50 ml | 200 ml |
| Međuspremnik RW1 | 50 ml | 200 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Recenzije
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