Uvod
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minuta. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + pročišćavanje, nucleic acid protection and in vitro translation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA from bacteria, yeast cells |
| Prijave | RT-PCR, Northern blot, poly-A + pročišćavanje, nucleic acid protection and in vitro translation |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Bacteria, yeast cells |
| Količina uzorka | Bacteria: <10^9；Yeast cells：<2 x10^7 |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤40 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (such as gvanidin hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 30 minuta
- Visoka čistoća – the purified RNA can be directly used in various downstream applications
- Visoki oporavak – RNA can be recovered at the level of PG
- Dobra ponovljivost – Tehnologija pročišćavanja stupca silikagela može svaki put dobiti idealne rezultate
- Široki spektar – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried wall-breaking enzymes and glass beads
Sadržaj kompleta
| Sadržaj | R418202 | R418203 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 250 |
| Glass Beads (0.1-0.6mm) | 30 g | 150 g |
| DNaza I | 600 μl | 5 x 600 μl |
| DNase pufer | 6 ml | 30 ml |
| Pufer za otapanje proteaze | 1.8 ml | 15 ml |
| Buffer ATL | 50 ml | 200 ml |
| Buffer PHC | 50 ml | 200 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Međuspremnik RW1 | 50 ml | 250 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
Buffer PHC should be stored at 2–8°C upon arrival. DNazu I treba čuvati na -20°C. Međutim, kratkotrajno skladištenje (DNase I do 1 tjedni, Buffer PHC up to 12 tjedni) na sobnoj temperaturi(15–25°C) ne utječe na njihovu izvedbu. The remaining kit components can be stored at roomtemperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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