Uvod
The HiPure Liquid RNA & miRNA Kit integrates phenol/gvanidin-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin stupac.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Prijave | qPCR / Rt-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection |
| Metoda pročišćavanja | Mini spin kolona |
| Tehnologija pročišćavanja | Silika tehnologija, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Krv, serum, plazma |
| Količina uzorka | 250μl |
| Volumen eluacije | ≥20μl |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100μg |
Načelo
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (do 100 µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Prednosti
- Visoka kvaliteta – ukupna RNA visoke čistoće može se izravno koristiti u raznim osjetljivim nizvodnim aplikacijama
- Fast – isolation of several samples can be completed in 40 minuta pomoću metode pročišćavanja kolonom
- Sigurnost – nije potrebna ekstrakcija fenol-kloroformom
- Osjetljiv – izravna liza krvi, plazma, a ostali uzorci bez izdvajanja leukocita
Sadržaj kompleta
| Sadržaj | R416302 | R416303 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 50 | 2 x 125 |
| MagZol LS Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Međuspremnik RW2* | 20 ml | 2 x 50 ml |
| Voda bez RNaze | 10 ml | 30 ml |
Skladištenje i stabilnost
MagZol LS Reagent should be stored at 2-8°C upon arrival. Međutim, kratkotrajno skladištenje (do 24 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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